Difference between revisions of "Trissolcus / Stinkbug Procedures"

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(Created page with "'''Protocol for Parasitoids and Egg Masses''' 1.) The egg mass comes in from the field, parasitoids emerge (or not) and the parasitoids go directly into ethanol. If the parasito...")
 
 
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'''Protocol for Parasitoids and Egg Masses'''
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[[File:Trissolcus.jpg]]
  
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1.) The egg mass comes in from the field, parasitoids emerge (or not) and the parasitoids go directly into ethanol. If the parasitoids all have the same emergence data, they can go into a single vial with as many barcodes as there are specimens.
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'''Excel Spreadsheet for Egg Mass and Parasitoid Data'''
  
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2.) The egg mass is glued (I used Elmer's glue) to a card mount which is on an insect pin, along with the barcode (as in the photo).
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[[File:TrissolcusBMSB_field_collections_datasheet.xls]]
  
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3.) At this point all of the data should be in the spreadsheet. Identifications should be made as best as can be done with confidence. Species-level identification can be done either in Newark, Delaware, or at the SEL in Washington.
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'''Key to relevant Platygastroid genera'''
  
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4.) The barcode for the specimens should face downward. The barcode for the egg masses should face upward so that one can easily see what "egg mass event" is in a given unit tray.
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[[File:Key_To_Platygastroid_Parasitoids_of_Stink_Bug_Eggs.pdf]]
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'''BMSB Egg Survey Protocol'''
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[[File:BMSBeggSurveyProtocolFeb2013.pdf]]
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'''Protocol for Egg Masses and Reared Parasitoids Post-Retrieval'''
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[[File:Post-Retrieval_Protocol.pdf]]
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== '''Contact for Identification of Parasitoids''' ==
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'''Elijah Talamas'''
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Research Entomologist, Hymenoptera Unit
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Systematic Entomology Lab, USDA/ARS
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c/o NMNH, Smithsonian Institution
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10th & Constitution Ave NW
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PO Box 37012 MRC-168
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Washington DC 20560
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Email: Elijah.Talamas@ars.usda.gov
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Phone: (202) 633-0998
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== '''Contact for Identification of Stink Bugs and Egg Masses''' ==
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'''Christine Dieckhoff'''
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USDA,REE,ARS,NAA,BIIR
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501 SOUTH CHAPEL STREET
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NEWARK, DE, 19713-3814
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Email: Christine.Dieckhoff@ars.usda.gov
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Phone: (302) 737-7330 ext. 242

Latest revision as of 20:17, 1 July 2013

Trissolcus.jpg

Excel Spreadsheet for Egg Mass and Parasitoid Data

File:TrissolcusBMSB field collections datasheet.xls

Key to relevant Platygastroid genera

File:Key To Platygastroid Parasitoids of Stink Bug Eggs.pdf

BMSB Egg Survey Protocol

File:BMSBeggSurveyProtocolFeb2013.pdf

Protocol for Egg Masses and Reared Parasitoids Post-Retrieval

File:Post-Retrieval Protocol.pdf

Contact for Identification of Parasitoids

Elijah Talamas

Research Entomologist, Hymenoptera Unit

Systematic Entomology Lab, USDA/ARS

c/o NMNH, Smithsonian Institution

10th & Constitution Ave NW

PO Box 37012 MRC-168

Washington DC 20560

Email: Elijah.Talamas@ars.usda.gov

Phone: (202) 633-0998


Contact for Identification of Stink Bugs and Egg Masses

Christine Dieckhoff

USDA,REE,ARS,NAA,BIIR

501 SOUTH CHAPEL STREET

NEWARK, DE, 19713-3814

Email: Christine.Dieckhoff@ars.usda.gov

Phone: (302) 737-7330 ext. 242

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